This webpage was produced as an assignment for Genetics 564, an undergraduate capstone course at UW-Madison.
Transcriptome
The central dogma, DNA to RNA to Protein, includes the step of transcribing DNA to RNA. The transcriptome of a cell or group of cells is what is being transcribed from DNA to RNA at a given time point. The transcriptome is constantly changing with the cell life, environment, and organismal changes/development.
Transcriptome of NF1
Here is an examination of hippocampus from 10 to 32 day old NF1 heterozygotes. NF1+/- mice develop learning and memory difficulties mimicking cognitive deficits in human neurofibromatosis. This heat map shows all of the genes that are either up regulated or down regulated in the hippocampus in NF1 heterozygotes at different stages in development.
Here is a segment of the above heat map analyzed for the gene's regular molecular function. This is taken from genes that are down regulated in the first half of development, and up regulated in the second half.
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Adenylate Cyclase is the enzyme that converts ATP to cAMP, which is important for cell-cell communication. NF1 is a modulator of adenylate cyclase, so it would make sense that the NF1+/- mice would have different levels of transcription of adenylate cyclase than that of the wild type.
References
Figure 1: The Central Dogma. https://genius.com/Biology-genius-the-central-dogma-annotated
Figure 2: Gene Expression Omibus. Neurofibromatosis and neurodevelopment.
Figure 3: PANTHER
Figure 4: GEO Profiles for Neurofibromatosis and neurodevelopment.
Figure 2: Gene Expression Omibus. Neurofibromatosis and neurodevelopment.
Figure 3: PANTHER
Figure 4: GEO Profiles for Neurofibromatosis and neurodevelopment.